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KMID : 0363320120330040465
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Journal of Korean Oriental Internal Medicine
2012 Volume.33 No. 4 p.465 ~ p.475
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Pro-apoptotic Effects of Sanguisorbae Radix Ethanol-Extracts on Two Cholangiocarcinoma Cell Lines, SNU-1079 and SNU-1196
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Kim Young-Chul
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Abstract
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Objectives : This study was performed to investigate the antineoplastic effect of ethanol extracts from Sanguisorbae Radix on cholangiocarcinoma cells that was established from biliary tract cancer tissue.
Materials and Methods : Two cholangiocarcinoma cell lines, SNU-1079 and SNU-1196, were studied. The mRNA expression of Caspase 3, 8, 9, Bcl-2, Bax, P53, and P21 was examined by RT-PCR. Cell viability was determined by MTT assay. The cell cycle was analyzed by flow cytometry and apoptosis by cell death detection ELISA kit.
Results : Proliferation of SNU-1079 and SNU-1196 was inhibited by Sanguisorbae Radix treatment in a dose-dependent manner. All cells treated with Sanguisorbae Radix showed increased dose- and time-dependent apoptosis. The expression of caspase 3, 8, 9, p53, and p21 was increased in all cells after the treatment of Sanguisorbae Radix. The expression of Bcl-2 was decreased in SNU-1196 and Bax expression was increased in all cells after the treatment of Sanguisorbae Radix.
Conclusions : These results suggest that Sanguisorbae Radix would be beneficial in the treatment of cholangiocarcinoma.
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KEYWORD
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Sanguisorbae Radix, cholangiocarcinoma, apoptosis
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